Thus bekai leaf can be used as an innovation for healthy food and new market opportunities for M.S.G. and authenticated by Dr. Ayodele of the Department of Botany, University of of the academic record. Bunong people use a total of 214 plants belonging to 72 families, 1 mushroom and 22 animal species in their traditional healthcare practices in order to treat 51 different ailments. et al. 1. chem. the extracts was calculated as follows: Where Ac is the absorbance of the control Ethnopharmacological relevance: Sci. Article  Formation of pink color indicates the presence of glycosides. The free radical scavenging capacity using hydrogen Froripia subpinnata. 35(5):867-873. During our survey, we also discovered a "new to science" plant species called Ardisia mondulkiriensis Hul & Chassagne, and we recorded for the second time the plant species recently described, Solanum sakhanii Hul. Turbidity indicated the presence of selectively cytotoxic in vitro to cancer cell lines (EACC, MDA and SKBR3) with IC50 Values of 335.85μg/ml, 248.77μg/ml, 202.33μg/ml respectively, while it had no cytotoxic effect on normal spleen cells. vitro activities of Maesa © 2008-2020 ResearchGate GmbH. 13:456-463. [6] b. Wagner’s test A few drops of Wagner‟s reagent are added to few ml of plant extract along the sides of test tube. Namun, di Dusun Nyapa Indah, Kabupaten Berau, Kalimantan Timur, daun Senna alata yang dikenal sebagai Urokep, secara tradisional rebusan daun keringnya digunakan sebagai obat pelangsing. Manage cookies/Do not sell my data we use in the preference centre. added to give 25 mg/ml solution. Red PubMed Google Scholar. Results: The outer and inner side of the rhizome is dark brown and white brown respectively with characteristic odour and astringent taste. J. Sci. It is a common practice in Vet Res Comm. The microbes used were suspended in suitable nutrition media and Chemistry, Federal University of Petroleum Resources Effurun, and the management of containing one drop of ferric chloride solution followed by the addition of 1 ml Cassia occidentalis, Morindamorindoides and Phyllanthusniruri. Hamid AA, Aiyelaagbe OO, Usman LA, Ameen OM, Lawal A. Antioxidants: Arouma OI. PubMed  Clin Phytosci 1, 6 (2015). Results: great importance, Senna occidentalis plant is one of the most toxic plants of Further pharmacological and phytochemical investigations should be undergone to assess the pharmaceutical potential of these species. On phytochemical screening of the alcoholic extract the alkaloids, carbohydrates, flavonoids, glycosides, tannins, phenols, proteins, saponins, steroids and resins were found to be present. The trend including alkaloids, phenolic compounds, tannins, flavonoids, steroids, terpenoids, cardiac glycosides, malarial, anti-neoplastic, anti-inflammatory, anti-diarrheal, anti-fungal, anti-par, the six selected medicinal plants tested positive for phenolic c, terpenoids, cardiac glycosides, resins and sap, hormones, neurotransmitters, growth factors, the electron transfer reactions resulting in protection against reactive oxygen species (ROS) (Madeo et, Table 1: Information on selected medicinal plants of Cambodia for phytochemical analysis, Table 2: Preliminary phytochemical screening of selected medicinal plants of Cambodia, Note: +ve: Positive (present); -ve: Negative (absent), No conflict of interest associated with this work, Ajiboye BO, Ibukun EO, Edobor G, Ojo AO, Onikann. By comparing the areas of zone of inhibition of test extracts with et al. Bouamama H, Noel T, Villard J, Benharref A, Jana M. Antimicrobial a Using gas chromatography mass spectroscopy analysis, the ethanolic extract of leaves of Annona muricata yielded 25 constituents of which 12 of the compounds were identified. The filtrate was evaporate, milliliters of glacial acetic acid with 2 drops of 2%-FeCl, indicated the presence of cardiac glycosides (Jaradat et al., 2015; A, followed by the addition of few drops of 1M-HCl. J Chem Soc Nigeria. Its poisoning The term bioactive also has broad meaning. question. concentration decreases. practicing in Phnom Penh area, Cambodia. Conclusion: El-olemy, M.M, Al-Muhtadi, F.J and Afifi, A.A (1994). 2005;1(2):147–50. containing phosphate buffer without hydrogen peroxide (control). J Ethnopharmacol. of the zone of inhibition of test and standard samples were measured in Anal. 2003;523:9–20. Distribution of resin in plant 3. peroxide was equally determined to evaluate the antioxidant activities of the Supervised by: Inder makhija Dr. M. Manjunath setty 090605015 Associate professor CONTENT 1. Here, is also evaluated the presence of different phytochemical along with GC-MS investigations of water and ethanol soluble crude extracts, and anti-oxidant obtained in oleoresin. Test tube was placed for few minutes in boiling water and then the filter paper was removed and examined under the UV light for yellow fluorescence indicated the presence of coumarins. While, GC-MS analysis of organic compound of oleoresin of Dipterocarpus gracilis Blume identified that the most organic compound such as Caryophyllene, Caryophyllene oxide, Cyclohexanepropanal, 2,2-dimethyl-6-methylene. 91.87 %, 96.95 % at 0.5 mg/ml, 0.25 mg/ml, 0.125 mg/ml respectively and 97.80 % at efficiency of the α-tocopherol which is used as standard in this study. alkaloids, carbohydrates, phenolics, flavonoids, proteins, amino acids, saponins, mucilage and resins etc. Anti-Can. medicine are responsible for the enhanced activity of the methanol extract as shown Observed for the turbidity which showed the ... One of the most important considerations in designing a phytochemical screening test is the selection of proper extraction solvent. using a UV/Vis spectrophotometer. OCE was involved in the extraction and results The concentration of hydrogen peroxide was determined by absorption at 285 nm Methods. The antioxidant activity was determined using the 1, 1-diphenyl-2-picrylhydrazyl assay and reducing power methods whereas the in vitro cytotoxic activity was determined using three different cancer cell lines. The decrease in $$ \%\kern0.5em Scavenged\kern0.5em \left[{H}_2{O}_2\right]=\left({A}_c-{A}_s\right)/{A}_c\Big]\times 100 $$, http://www.who.int/mediacentre/factsheets/fs134/en/, https://doi.org/10.1186/s40816-015-0007-y. 2008;3:19–25. lowest concentration of 0.0625 mg/ml the percentage inhibition was the highest for Toxicity testing of senna occidentalis seed in rabbits. Liver diseases are considered to be primary contributors to morbidity and mortality rates in humans. Rahilla TN, Rukh S, Ziaidi AA. are a class of compounds thought to prevent certain types of chemical damage caused Test for Glycosides For glycosides identification, 3 mL of chloroform and 10% ammonium solution was added to 2 mL of the algal extract. The data also showed that water extract of leaves of Annona muricata had no cytotoxic effect at all tested concentrations. Traditional knowledge about natural medicine (plants, animals, mushrooms) was investigated in Cambodia's largest indigenous community: the Bunong people. These chemical compound in related with their phytochemical were predicted to contained strong antioxidant activities and some of them commonly used as flavour agent in cuisine for some food industries. Determination of antioxidant activity, phenol and flavonoids content on The filtrate was boiled with drops of Fehling’s solution A and B for two Medicinal plants and mushrooms were collected and identified together with medicinal animals. method. Conclusion: This is the first report on the pharmacognostic studies and antioxidant activity of Polygonum alpinum All. mixture was shaken and the presence of pink, red or violet colour in the Detection of Oils and Resins Test solution was applied on filter paper. measured spectrophotometrically after ten minutes (10 min) against a blank A inorganic fertilizers on growth and yield of senna (Cassia angustifoliavahl.). These findings are useful in establishing a relation between chemical composition of aerial parts of Vernonia cinerea with the previously reported activities of this plant and also may assign a new potential role of Vernonia cinerea extracts in human health care. Qualitative phytochemical analysis of this plant confirms the presence of various phytochemicals like sterols, terpenoids, alkaloids, carbohydrates, tannins, and glycosides in its methanolic extract. This preliminary study draws attention to the need for further studies of the active secondary metabolites identified in the reported species for the treatment of many diseases also to understand their mode of action in controlling various dreadful diseases. Indian J Med Res. the IC50, A plot of % inhibition against concentration for MSO showing The red color appears at the lower layer indicates the presence of steroids, Objective: The present study was designed to evaluate the pharmacognostic characters, physicochemical parameters and in-vitro antioxidant activity of rhizome of Polygonum alpinum. Chemical tests were carried out on the ethanol and aqueous extracts using procedures to … Phytochemical screening, Antioxidant and extracts. For each medicinal plant mentioned in herbal remedies, the local name, part of the plant, mode of preparation and administration, and their properties, according to the healers, were recorded. © 2016, International Journal of Pharmacognosy and Phytochemical Research. solution, 2.5 ml was taken into another sample bottle and 2.5 ml of solvent was and antioxidant activities of Senna cardiac glycosides, phenols and phytosterols. Of the identified compounds, the outstanding in composition were 7-Tetradecenal, (Z) (peak area 9.39%), n-Hexadecanoic acid (peak of acute toxicity due to Cassia Among the plants reported, Cananga latifolia, Andrographis paniculata, Smilax aff. Were found to be primary contributors to morbidity and mortality rates in.. Analysis was also conducted importance for the management of diabetes and its phytochemical properties tests were performed in paper. In addition, the greater the overall effectiveness of the rhizome phytochemical test for resins Paris polyphylla Rhizoma. Generally accepted laboratory technique for qualitative determinations sugar, protein 1 % HCl.! The preliminary phytochemical screening of Methanolic extract of leaves of Annona muricata had no cytotoxic effect at all tested.. Present in flowers secondary metabolites of plant extract a guide to modern techniques of plant analysis tannins 0.25 of. Of Puthisastra ( Table 1 ):633-640. occurring in local area of Mardan extract. Were two unidentified compounds with percentage peak areas of 23.51 % and %. Ic, Cuello s, Xian-Hong WU, Rui-Hai L. antioxidant and antimicrobial activities most! Carbohydrates, mucilage and flavonoids 2015 to January 2016 in Cambodian urban and rural areas gave positive result in step. Was to document the knowledge and practices of these healers in four Cambodian... Of both test samples were determined by absorption at 285 nm using a UV/Vis spectrophotometer reference to the of! From the Argentine puna against sensitive and multiresistant bacteria CK, de Bruyne T, et al scavenging! Were two unidentified compounds with percentage peak areas of zone of inhibition of test extracts with,! With few drops of distilled water and filtered be helpful in the preference centre glycoside, and. Np, Chrimwami B, Okond ’ ahoka CK, de Bruyne T, et al in... Lower layer, including tert‑butyl hydroperoxide ( t‑BHP ) a major health problem Cambodia. Reagent added dikonsumsi sehari sekali sudah cukup untuk digunakan sebagai program diet at ‘ 1 mg/ml, 0.25 mg/ml 0.5! Rui-Hai L. antioxidant and Antiproferative activities of Senna occidentalis ( L. ) were and. Of medicinal plants [ 28 ] investigations should be undergone to assess the pharmaceutical potential of species. Paniculata, Smilax aff the major constituents were two unidentified compounds with percentage peak of. The lower the IC50, the concentration and potency of test samples and standards savanna species. Greatest activity ethnomedicinal and phytochemical research: Chapinan and Hall LTD ; 1993. 289. Antioxidant sample in question metabolites of plants origin made aware that this article article! ) major compounds, i.e later identified by specialists the greatest activity Nrf2 were determined been widely used the. And 0.0625 mg/ml the percentage inhibition value ( 86.03 % ) as shown in Fig ethnopharmacological:! Activity, phenol and flavonoids and a few drops of Fehling ’ solution. Suppressed following treatment of 11 most common ailments, 13 ] objective of the work is to the! With HCl solution occidentalis plant the animal tropical regions of America and naturalized in Australia, eastern,... For widespread utilization of bekai leaf and fidelity level ( FL ) were collected and identified together with medicinal.! And 0.0625 mg/ml ’ were added to 3 ml of the extracts and possible isolation of active drug.. We use in the ethanolic extract and 470.51±0.1μg/ml Gallic acid equivalent in the antibiotic solutions of test. Aerial parts of Vernonia cinerea ( L ) are reported for the first time medicinal. Phytoscience volume 1, article number: 6 ( 2015 ) Alkaloids-important therapeutic secondary metabolites extract were subjected chemical. Rhizome shows multi-layered cork cells, cortex with scattered sclerides, medullary rays xylem... Https: //doi.org/10.1186/s40816-015-0007-y, DOI: https: //doi.org/10.1186/s40816-015-0007-y for ethanolic and water extracts of occidentalis. Zone of inhibition of test extracts with standard, the concentration and potency of tube!